Saturday, October 22, 2011

Science tool for a Molecular Biology Laboratory

To start with, when you begin equipping a molecular biology lab, you need the usual test tubes, beakers, desiccators, pipettes with fillers, and flasks, together with all the essential clamps, stoppers, frames and supports.

The basic furniture will need to consist of enough worktop space, computer station/s, chemical and biological storehouse cabinets, maybe a filtered, controlled environment (heating & cooling, and humidity control). In some cases way control, a gray room and clean room conditions may even be necessary.

Clamp Meter

Since this subject of science deals with organic materials from living cell tissues, it stands to conjecture that temperatures are critical. Most laboratories use refrigerators and freezers, and sometimes surface mounted refrigeration units. Some procedures may need temperatures to be reduced to as low as -86A deg. C.

Centrifuges and microcentrifuges may need to stand on refrigerated and unrefrigerated tabletops for separate purposes.

The heating devices may include a microwave, Bunsen burners, and/or hot plates.

There are a number of calibrated and estimation devices, apart from the glassware, that will be needed in a laboratory, such as certain plastic ware, micro balances and scales, pH meters, moisture meters and thermometers.

Microscopes or electron microscopes acceptable to the study being done, together with camera port and link to computer. These are a part of every laboratory.

There is a large number of apparatus and tool generally determined essential for a molecular biology laboratory. Tissue processing for Dna identification is often an prominent part of molecular biology and many processes need the tool for this, depending on the extent of Dna study and genomics. Here is some of the general apparatus likely to be needed:

* Co2 incubator for cell cultures.
* Vacuum pumps.
* Liquid nitrogen tank.
* Water bath.
* Magnetic stirrer.
* Spectrophotometer.
* Chromatograph.
* Vortex rotators and platform shakers.
* Autoclaves and sterilizers.
* Electrophoresis room and gel casting and documentation chamber.
* Uv Transilluminator.
* Gradient and acceptable thermocycler to amplify segments of Dna.

Then we come to the question of security and security of the environment and of the citizen who work in the laboratory; and most of the following will normally be necessary:
Clothing such as:

* Aprons and lab coats.
* Coveralls.
* Footwear, such as shoe covers.
* Head covers, bouffant caps and face covers, masks.

Gloves:

* Heavy weight latex gloves.
* Thin, disposable latex gloves.
* Nitrile gloves.
* Cotton gloves.
* Vinyl gloves.

For eye security there is a great collection of security glasses, clear and tinted for lazer and Uv work, as well as masks and face shields with and without respirators for security against splashing, fumes and gasses and flying objects. It is prominent in a laboratory to setup an eyewash middle point for emergencies.

Last but not least is all the tool needed for cleaning and sterilizing the glassware, apparatus and even the clothing and furniture used in the laboratory. The cleaning of these items is not always straight forward, and some special procedures need to be followed. You need to have specially designed scrubbers and brushes as well as suitable cleaning fluids and preparations, among other things.

Science tool for a Molecular Biology Laboratory

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